Integrated Methods in Protein Biochemistry: Part A, Volume 677, the latest release in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on topics such as DNA and protein engineering to create protein bioswitches with new functions, Interaction and cross-talk of prelamin A with integral membrane zinc metalloproteases, An experimental protocol to study lipid transfer proteins, Synthesis of small heat shock proteins, Druggable p-p interacting sites for Co-chaperone DNAJA1 and its partner proteins, An experimental protocol for glycoconjugate analysis, Methods for proximity-based biotinylation combined with Mass Spectrometry, and more. Additional chapters cover Synthetic antibody fragments as conformational sensors of protein activation and trafficking, Expression, purification, functional analysis and crystallization of Rag GTPase, Purification of bacterial transcription elongation complexes by photoreversible immobilization, Inhibition of c-Myc-MAX heterodimerization, Fluorogenic RNA aptamers to probe transcription by multi-subunit RNA polymerases, and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology series Updated release includes the latest information on Integrated Methods in Protein Biochemistry INDICE: 1. DNA and protein engineering to create protein bioswitches with new functionsStewart Loh2. Interaction and cross-talk of prelamin A with integral membrane zinc metalloproteasesSusan Michaelis3. An experimental protocol to study lipid transfer proteinsHaijia Yu4. Synthesis of small heat shock proteinsMatthew Pratt5. Druggable p-p interacting sites for Co-chaperone DNAJA1 and its partner proteinsGuang-Yu Yang6. An experimental protocol for glycoconjugate analysisYifa Zhou7. Methods for proximity-based biotinylation combined with Mass SpectrometryRalph Kehlenbach8. Synthetic antibody fragments as conformational sensors of protein activation and traffickingArun K. Shukla9. Expression, purification, functional analysis and crystallization of Rag GTPaseKuang Shen10. Purification of bacterial transcription elongation complexes by photoreversible immobilizationEric Strobel11. Inhibition of c-Myc-MAX heterodimerizationNeha Garg12. Fluorogenic RNA aptamers to probe transcription by multi-subunit RNA polymerasesMarkus C. Wahl13. Phosphorylation and subcellular localization of human phospholipase A1Atsushi Yamashita14. Integrated methods in studying light-sensitive proteinsLars-Oliver Essen15. Structure and biochemistry of thermophilic proteinAnkan Banerjee16. Using hydrogen/deuterium exchange mass spectrometry to probe protein structureGert Bange17. Nanopore force spectroscopy to probe structural dynamics of proteinsSonja Schmid18. Structural analysis of knotted proteinsShang-Te Danny Hsu19. Mutation-induced structural changes in the SARC-CoV2 spike proteinShang-Te Danny Hsu20. Preparing metabolite capped RNA substrates using RNA polymerasesJeremy G. Bird
- ISBN: 978-0-323-99266-4
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 412
- Fecha Publicación: 01/10/2022
- Nº Volúmenes: 1
- Idioma: Inglés