This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. Provides the authority and expertise of leading contributors from an international board of authorsPresents the latest release in the Methods in Enzymology series INDICE: 1. Genetic labeling of extracellular vesicle exosomes for studying biogenesis and uptake in living mammalian cells Biao Lu 2. Fluorescent Labeling of Extracellular Vesicles Thomas Gaborski 3. Isolation of extracellular vesicles from lymph Laura Santambrogio 4. Transgenic rats for tracking body fluid/tissue-derived extracellular vesicles Takahiro Ochiya 5. Isolation of amniotic extracellular vesicles Ramkumar Menon 6. Urinary extracellular vesicle isolation Antonio De Maio 7. Immunocapture-based ELISA to Characterize and Quantify Extracellular Vesicles in Both Cell Culture Supernatants and Body Fluids Stefano Fais 8. Extracellular vesicle analysis by nano FACS John P. Nolan 9. Quantification of mtDNA in extracellular vesicles Beata Soltesz 10. Extracellular vesicle isolation from 3D cell cultures Irina Nazarenko 11. Lipidomic profile of extracellular vesicles Jessy Deshane 12. Imaging extracellular vesicles using fluorescent microscpe Ema Cocucci 13. Single extracellular vesicle analysis Tobias Tertel 14. Isolation of circulating extracellular vesicles Emmanuel Dias-Neto 15. Live extracellular vesicles tracking in zebrafish Jacky Goetz 16. TEM-assisted characterization of extracellular vesicles Alain R. Brisson
- ISBN: 978-0-12-820662-1
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 412
- Fecha Publicación: 01/11/2020
- Nº Volúmenes: 1
- Idioma: Inglés