Mechanisms of DNA Recombination and Genome Rearrangements: Intersection Between Homologous Recombination, DNA Replication and DNA Repair

Mechanisms of DNA Recombination and Genome Rearrangements: Intersection Between Homologous Recombination, DNA Replication and DNA Repair, Volume 601, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Homologous genetic recombination remains the most enigmatic process in DNA metabolism. The molecular machines of recombination preserve the integrity of the genetic material in all organisms and generate genetic diversity in evolution. The same molecular machines that support genetic integrity by orchestrating accurate repair of the most deleterious DNA lesions, however, also promote survival of cancerous cells and emergence of radiation and chemotherapy resistance. This two-volume set offers a comprehensive set of cutting edge methods to study various aspects of homologous recombination and cellular processes that utilize the enzymatic machinery of recombination The chapters are written by the leading researches and cover a broad range of topics from the basic molecular mechanisms of recombinational proteins and enzymes to emerging cellular techniques and drug discovery efforts. contributions by the leading experts in the field of DNA repair, recombination, replication and genome stabilitydocuments cutting edge methods INDICE: Using the Flp recombinase to induce site-specific nicksLotte BjergbaekSynthesis from D-loopsWolf-Dietrich HeyerBacteriophage T4 system as a model for recombination/replication interfaceScott MorricalInducing and detecting mitotic DNA synthesis at difficult-to-replicate lociIan HicksonReporter system to study the RNA-DNA recombinationFrancesca StoriciAssaying recombination and mutagenesis at DNA nicksNancy MaizelsRecovery of alternative end-joining repair products from Drosophila embryosMitch McVeyHigh-throughput analysis of DNA break-induced chromosome rearrangements by amplicon sequencingSteven RobertsAnalysis of mutagenesis associated with recombinationJim HaberInvestigation of break-induced replication in yeastAnna MalkovaDissecting the dual endo- and exonuclease activity of MRE11 (MRN) with small moleculesJohn Tainer and Davide Moiani RAD52 inhibitionAshley SpiesStructure-guided, fragment-based drug discovery against RAD51 recombinase: targeting a protein-peptide interfaceThomas BlundellMolecular intermediates of recombination in meiosis (methods, like 2D, and others)Neil HunterS1-seq method for mapping DSB resection endpointsScott KeneyMethods for studying the roles of the Sgs1-Top3-Rmi1 helicase/decatenase complex in meiotic recombination intermediate metabolismMichael LichtenLive cell imaging of heterochromatin repair in Drosophila cellsIrene ChioloAnalysis of meiotic double-strand break initiation in mammals Galina Petukhova and Dan Camerini-Otero

• ISBN: 978-0-12-813979-0
• Editorial: Academic Press
• Encuadernacion: Cartoné
• Páginas: 412
• Fecha Publicación: 01/02/2018
• Nº Volúmenes: 1
• Idioma: Inglés