Mechanisms of DNA Recombination and Genome Rearrangements: Methods to Study Homologous Recombination

Mechanisms of DNA Recombination and Genome Rearrangements: Methods to Study Homologous Recombination, Volume 600, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Homologous genetic recombination remains the most enigmatic process in DNA metabolism. The molecular machines of recombination preserve the integrity of the genetic material in all organisms and generate genetic diversity in evolution. The same molecular machines that support genetic integrity by orchestrating accurate repair of the most deleterious DNA lesions, however, also promote survival of cancerous cells and emergence of radiation and chemotherapy resistance. This two-volume set offers a comprehensive set of cutting edge methods to study various aspects of homologous recombination and cellular processes that utilize the enzymatic machinery of recombination The chapters are written by the leading researches and cover a broad range of topics from the basic molecular mechanisms of recombinational proteins and enzymes to emerging cellular techniques and drug discovery efforts. Contributions by the leading experts in the field of DNA repair, recombination, replication and genome stabilityDocuments cutting edge methods INDICE: 1. DSB processing in yeast Loraine Symington 2. Purification of DNA end resection enzymes Petr Ceijka 3. Biochemistry of DNA end resection Petr Ceijka 4. Visualization of telomere movement and interchromosomal homology searches in ALT Roger Greenberg 5. Pre-steady state kinetic methods for investigating the DNA exonuclease activity of Mre11 Scott Nelson 6. Production of large quantities of human RAD51 Maria Spies 7. Determining the RAD51 nucleoprotein filamentous structure and mechanism by CryoEM Hong-Wei Wang 8. Single-molecule TIRF analysis of human RAD51 nucleation Maria Spies 9. TIRF-based single-molecule detection of the RecA presynaptic filament dynamics Sung Hyun Kim 10. Archaeal (Sulpholobus) RadA and its paralogs Cynthia Haseltine 11. 4-strand DNA strand exchange Alexander Mazin 12. Purification and biochemistry of DMC1 recombinase Doug Bishop 13. Single-molecule TIRF analysis of yeast Rad51 nucleoprotein disassembly by helicases Sua Myong 14. The recombination mediator BRCA2: architectural plasticity of recombination intermediates revealed by single molecule imaging (SFM/TIRF) Claire Wyman 15. Visualisation and quantitation of BRCA2/RAD51 dynamics in cells Roland Kanaar 16. RPA - DNA curtains Eric Greene 17. Single-molecule studies of ssDNA binding proteins Taekjip Ha 18. BRCA2 as recombination mediator Aura Carreira 19. Ustilago maydis Brh2 as a model for human BRCA2 Bill Holloman 20. Purification and analysis of holiday junction resolvases Stephen West 21. Structural analysis of HJ resolving enzyme GEN1 David Lilley 22. The preparation of four-stranded recombination intermediates for use in the HJ resolution reactions  Stephen West

• ISBN: 978-0-12-814429-9
• Editorial: Academic Press
• Encuadernacion: Cartoné
• Páginas: 412
• Fecha Publicación: 19/01/2018
• Nº Volúmenes: 1
• Idioma: Inglés