Single-Molecule Enzymology, Part A, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in single-molecule enzymology, and includes sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, nanopores, and tethered particle motion. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods in single-molecule enzymologyContains sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, nanopores, and tethered particle motion INDICE: 1. HelicaseMichelle Wang2. OT & fluorescenceErwin Peterman and Gijs Wuite 3. HelicasesTaekjip Ha4. Super-resolution imaging of synapses Paul R. Selvin and Sang Hak Lee 5. FIB modify cantileversTom Perkins6. How to measure separations and angles between intra-molecular fluorescent markersJames Spudich, Henrik Flyvbjerg, Kim Mortensen and Jongmin Sung 7. How to measure load-dependent kinetics of individual motor molecules without a force clampJames Spudich, Henrik Flyvbjerg, Kim Mortensen and Jongmin Sung 8. Magnetic tweezer - CRISPRRalf Seidel9. Single-molecule enzymologyH. Peter Lu10. MT - helicases/replicationVincent Croquette11. MT & fluorescence - RNA polymeraseTerence Strick12. Direct fluorescent imaging of DNA helicase translocation and DNA unwindingStephen C. Kowalczykowski13. Multi-color FRET Sungchul Hohng14. Dynamics of Hsp90 and nucleotides by multi-color single molecule FRETThorsten Hugel15. Labeling Biomolecules with Fluorophores in Cell Extracts for Single Molecule ExperimentsAaron Hoskins16. Single Molecule Cluster Analysis (SiMCAn)Nils G. Walter17. smFRET via confocal microscopy - topoisomerasesDagmar Klostermeier18. smFRET - conformational dynamics (MMR)Keith Weninger19. smFRET/zero-mode waveguides (ZMWs), vbFRETRuben Gonzalez20. Single molecule imaging with one color fluorescenceSua Myong21. Single-molecule kinetics of multicomponent systemsMaria Spies22. smFRET - DNA polymeraseAchillefs Kapanidis and Johannes Hohlbein 23. Single-Molecule Fluorescence Studies of Fast Protein FoldingVictor Munoz24. iSCAT - cytoskeletal motorsPhilipp Kukura25. Emerging Nanopore applications/discoveriesAmit Meller26. Single molecule technique using nanopores (SPRNT)Jens H. Gundlach27. Tethered particle motion - DNA looping & TFLaura Finzi28. Enzyme measurements in microwellsDavid Walt29. Assembly and characterization of Rad51/RecA presynaptic complexes using DNA curtainsEric C. Greene30. DNA curtains - diffusion of MMR proteinsIlya J. Finkelstein31. Optical trappingAhmet Yildiz32. Membrane protein association reactions in membranesJanice Robertson33. Integrating optical tweezers, DNA tightropes and single molecule fluorescence imaging: pitfalls and trapsNeil Kad and Jingyu Wang 34. Membrane protein reconstitution in nanodiscs/liposomes Nikos Hatzakis35. Force dependent kinetics Sanjeevi Sivasankar36. smFRET studies of modified nucleosomes Michael Poirier
- ISBN: 978-0-12-809267-5
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 412
- Fecha Publicación: 01/11/2016
- Nº Volúmenes: 1
- Idioma: Inglés