Antiviral resistance in plants: methods and protocols

Studies related to pathogen-mediated virus resistance in plants were instrumental in providing some of the historical observations which ultimately led to the vital discovery of double-stranded RNA (dsRNA)-induced gene silencing or RNA interference (RNAi), which has since revolutionized research on plant-virusinteractions. In Antiviral Resistance in Plants: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study the phenomenon of RNA silencing in relation to viral infections of plants. These include methods and techniques for the isolation and quantitative/qualitative analyses of plant small 21-24 nucleotide RNAs such as small interfering RNAs (siRNAs) and microRNAs (miRNAs) as well as the analysis and manipulation of virus-induced gene silencing (VIGS) in both monocotyledonous anddicotyledenous plants and the use of hairpin RNA (hpRNA) transgenes. Writtenin the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Antiviral Resistance in Plants: Methods and Protocols seeks to aid scientists in the further study of this crucially importantbotanical trait. INDICE: 1. An Historical Overview of RNAi in Plants John A. Lindbo 2. RNASilencing and Antiviral Defense in Plants Claire Agius, Andrew Eamens, Tony Millar, John M. Watson, and Ming-Bo Wang 3. Biological Chemistry of Virus-encoded Suppressors of RNA Silencing: An Overview Rustem T. Omarov and Herman B. Scholtof 4. Virus-Induced Gene Silencing Using Begomovirus Satellite Molecules Xueping Zhou and Changjun Huang 5. Strategies for Viral Cross Protection in Plants Changyong Zhou and Yan Zhou 6. Tobacco Rattle Virus (TRV)-Based Virus-Induced Gene Silencing Shawn Bachan and S.P. Dinesh-Kumar 7. Virus-Induced Gene Silencing in Hexaploid Wheat Using Barley Stripe Mosaic Virus Vectors Steven R. Scofield and Amanda S. Brandt 8. Characterization of Plant Virus-Encoded Gene Silencing Suppressors Hajime Yaegashi, Masamichi Isogai, and Nobuyuki Yoshikawa 9. Generation of Plant Small RNA cDNA Libraries for High-Throughput Sequencing Qian-Hao Zhu and Chris Helliwell 10. Modelling Antiviral Resistance in Plants Robert S. Anderssen and Peter M. Waterhouse 11. Isolation and Detection of Small RNAs from Plant Tissues Neil A. Smith and Andrew L. Eamens 12. Isolationand Analysis of Small RNAs from Virus-Infected Plants Shaun J. Curtin, Agustin Zsögön, John M. Watson, and Peter M. Waterhouse 13. Use of Hairpin RNA Constructs for Engineering Plant Virus Resistance Neena Mitter and Ralf G. Dietzgen14. Artificial MicroRNAs for Plant Virus Resistance Jing Qu, Jian Ye, and Rongxiang Fang 15. Isolation and Biochemical Analysis of Plant Small RNAs H. Alexander Ebhardt, Mariana Oviedo Ovando, and Peter J. Unrau 16. Isolation and Analysis of Plant RNA-Dependent RNA Polymerases Julien Curaba 17. Plant Viroids: Isolation, Characterization/Detection, and Analysis Robert A. Owens, Teruo Sano, and Nuria Duran-Vila 18. Structural and Functional Analysis of CMV Satellite RNAs in RNA Silencing Hanako Shimura and Chikara Masuta 19. Virus-Induced Gene Silencing in Soybean Aardra Kachroo and Said Ghabrial 20. Chimeric, Infectious, and Stable Virus Transcripts to Study RNA Silencing in ‘Dark Green’ Islands Robin M. MacDiarmid 21. Rolling Circle Amplification-Mediated Hairpin RNA (RMHR) Library Construction in Plants Lei Wang and Yun-Liu Fan 22. The Interaction Between Plant Viroid-Induced Symptoms and RNA Silencing Vicente Pallas, German Martinez, and Gustavo Gomez 23. Identification of Small Molecule Inhibitors of Tomato Bushy Stunt Virus (TBSV) Replication Zsuzsanna Sasvari and Peter D. Nagy 24. Expression of Dominant-Negative Mutants to Study Host Factors Affecting Plant Virus Infections Daniel Barajas and Peter D. Nagy Hajime Yaegashi,Masamichi Isogai, and Nobuyuki Yoshikawa 9. Generation of Plant Small RNA cDNA Libraries for High-Throughput Sequencing Qian-Hao Zhu and Chris Helliwell 10. Modelling Antiviral Resistance in Plants Robert S. Anderssen and Peter M. Waterhouse 11. Isolation and Detection of Small RNAs from Plant Tissues Neil A. Smith and Andrew L. Eamens 12. Isolation and Analysis of Small RNAs from Virus-Infected Plants Shaun J. Curtin, Agustin Zsögön, John M. Watson, and Peter M.Waterhouse 13. Use of Hairpin RNA Constructs for Engineering Plant Virus Resistance Neena Mitter and Ralf G. Dietzgen 14. Artificial MicroRNAs for Plant Virus Resistance Jing Qu, Jian Ye, and Rongxiang Fang 15. Isolation and Biochemical Analysis of Plant Small RNAs H. Alexander Ebhardt, Mariana Oviedo Ovando, and Peter J. Unrau 16. Isolation and Analysis of Plant RNA-Dependent RNA Polymerases Julien Curaba 17. Plant Viroids: Isolation, Characterization/Detection,and Analysis Robert A. Owens, Teruo Sano, and Nuria Duran-Vila 18. Structuraland Functional Analysis of CMV Satellite RNAs in RNA Silencing Hanako Shimuraand Chikara Masuta 19. Virus-Induced Gene Silencing in Soybean Aardra Kachrooand Said Ghabrial 20. Chimeric, Infectious, and Stable Virus Transcripts to Study RNA Silencing in ‘Dark Green’ Islands Robin M. MacDiarmid 21. Rolling Circle Amplification-Mediated Hairpin RNA (RMHR) Library Construction in Plants Lei Wang and Yun-Liu Fan 22. The Interaction Between Plant Viroid-Induced Symptoms and RNA Silencing Vicente Pallas, German Martinez, and Gustavo Gomez 23. Identification of Small Molecule Inhibitors of Tomato Bushy Stunt Virus (TBSV) Replication Zsuzsanna Sasvari and Peter D. Nagy 24. Expression of Dominant-Negative Mutants to Study Host Factors Affecting Plant Virus Infections Daniel Barajas and Peter D. Nagy Neil A. Smith and Andrew L. Eamens 12. Isolation and Analysis of Small RNAs from Virus-Infected Plants Shaun J. Curtin, Agustin Zsögön, John M. Watson, and Peter M. Waterhouse 13. Use of Hairpin RNA Constructs for Engineering Plant Virus Resistance Neena Mitter and Ralf G. Dietzgen 14. Artificial MicroRNAs for Plant Virus Resistance Jing Qu, Jian Ye, and RongxiangFang 15. Isolation and Biochemical Analysis of Plant Small RNAs H. Alexander Ebhardt, Mariana Oviedo Ovando, and Peter J. Unrau 16. Isolation and Analysis of Plant RNA-Dependent RNA Polymerases Julien Curaba 17. Plant Viroids: Isolation, Characterization/Detection, and Analysis Robert A. Owens, Teruo Sano, andNuria Duran-Vila 18. Structural and Functional Analysis of CMV Satellite RNAsin RNA Silencing Hanako Shimura and Chikara Masuta 19. Virus-Induced Gene Silencing in Soybean Aardra Kachroo and Said Ghabrial 20. Chimeric, Infectious, and Stable Virus Transcripts to Study RNA Silencing in ‘Dark Green’ Islands Robin M. MacDiarmid 21. Rolling Circle Amplification-Mediated Hairpin RNA (RMHR) Library Construction in Plants Lei Wang and Yun-Liu Fan 22. The Interaction Between Plant Viroid-Induced Symptoms and RNA Silencing Vicente Pallas, German Martinez, and Gustavo Gomez 23. Identification of Small Molecule Inhibitors of Tomato Bushy Stunt Virus (TBSV) Replication Zsuzsanna Sasvari and Peter D. Nagy 24. Expression of Dominant-Negative Mutants to Study Host Factors Affecting Plant Virus Infections Daniel Barajas and Peter D. Nagy Neena Mitter and Ralf G. Dietzgen 14. Artificial MicroRNAs for Plant Virus Resistance Jing Qu, Jian Ye, and Rongxiang Fang 15. Isolation and Biochemical Analysis of Plant Small RNAs H. Alexander Ebhardt, Mariana Oviedo Ovando, and Peter J. Unrau 16. Isolation and Analysis of Plant RNA-Dependent RNA Polymerases Julien Curaba 17. Plant Viroids: Isolation, Characterization/Detection, and Analysis Robert A. Owens, Teruo Sano, and Nuria Duran-Vila 18. Structural and Functional Analysis of CMV Satellite RNAs in RNA Silencing Hanako Shimura and Chikara Masuta 19. Virus-Induced Gene Silencing in Soybean Aardra Kachroo and Said Ghabrial 20. Chimeric, Infectious, and Stable Virus Transcripts to Study RNA Silencing in ‘Dark Green’ Islands Robin M. MacDiarmid 21. Rolling Circle Amplification-Mediated Hairpin RNA (RMHR) Library Construction in Plants Lei Wang and Yun-Liu Fan 22. The Interaction Between Plant Viroid-Induced Symptoms and RNA Silencing VicentePallas, German Martinez, and Gustavo Gomez 23. Identification of Small Molecule Inhibitors of Tomato Bushy Stunt Virus (TBSV) Replication Zsuzsanna Sasvariand Peter D. Nagy 24. Expression of Dominant-Negative Mutants to Study Host Factors Affecting Plant Virus Infections Daniel Barajas and Peter D. Nagy Jing Qu, Jian Ye, and Rongxiang Fang 15. Isolation and Biochemical Analysis of Plant Small RNAs H. Alexander Ebhardt, Mariana Oviedo Ovando, and Peter J. Unrau 16. Isolation and Analysis of Plant RNA-Dependent RNA Polymerases Julien Curaba17. Plant Viroids: Isolation, Characterization/Detection, and Analysis RobertA. Owens, Teruo Sano, and Nuria Duran-Vila 18. Structural and Functional Analysis of CMV Satellite RNAs in RNA Silencing Hanako Shimura and Chikara Masuta 19. Virus-Induced Gene Silencing in Soybean Aardra Kachroo and Said Ghabrial 20. Chimeric, Infectious, and Stable Virus Transcripts to Study RNA Silencing in ‘Dark Green’ Islands Robin M. MacDiarmid 21. Rolling Circle Amplification-Mediated Hairpin RNA (RMHR) Library Construction in Plants Lei Wang and Yun-Liu Fan 22. The Interaction Between Plant Viroid-Induced Symptoms and RNA Silencing Vicente Pallas, German Martinez, and Gustavo Gomez 23. Identification of Small Molecule Inhibitors of Tomato Bushy Stunt Virus (TBSV) Replication Zsuzsanna Sasvari and Peter D. Nagy 24. Expression of Dominant-Negative Mutants to Study Host Factors Affecting Plant Virus Infections Daniel Barajas and Peter D. Nagy H. Alexander Ebhardt, Mariana Oviedo Ovando, and Peter J. Unrau 16. Isolation and Analysis of Plant RNA-Dependent RNA Polymerases Julien Curaba 17. Plant Viroids: Isolation, Characterization/Detection, and Analysis Robert A. Owens, Teruo Sano, and Nuria Duran-Vila 18. Structural and Functional Analysis of CMV Satellite RNAs in RNA Silencing Hanako Shimura and Chikara Masuta 19. Virus-Induced Gene Silencing in Soybean Aardra Kachroo and Said Ghabrial 20. Chimeric, Infectious, and Stable Virus Transcripts to Study RNA Silencing in ‘Dark Green’ Islands Robin M. MacDiarmid 21. Rolling Circle Amplification-Mediated Hairpin RNA (RMHR) Library Construction in Plants Lei Wang and Yun-Liu Fan 22. The Interaction Between Plant Viroid-Induced Symptoms and RNA Silencing VicentePallas, German Martinez, and Gustavo Gomez 23. Identification of Small Molecule Inhibitors of Tomato Bushy Stunt Virus (TBSV) Replication Zsuzsanna Sasvariand Peter D. Nagy 24. Expression of Dominant-Negative Mutants to Study Host Factors Affecting Plant Virus Infections Daniel Barajas and Peter D. Nagy Julien Curaba 17. Plant Viroids: Isolation, Characterization/Detection, and Analysis Robert A. Owens, Teruo Sano, and Nuria Duran-Vila 18. Structural and Functional Analysis of CMV Satellite RNAs in RNA Silencing Hanako Shimura and ChikaraMasuta 19. Virus-Induced Gene Silencing in Soybean Aardra Kachroo and Said Ghabrial 20. Chimeric, Infectious, and Stable Virus Transcripts to Study RNA Silencing in ‘Dark Green’ Islands Robin M. MacDiarmid 21. Rolling Circle Amplification-Mediated Ha

• ISBN: 978-1-61779-881-8
• Editorial: Humana
• Encuadernacion: Cartoné
• Páginas: 360
• Fecha Publicación: 18/07/2012
• Nº Volúmenes: 1
• Idioma: Inglés