Fundamentals of Recombinant Protein Production, Purification and Characterization

Production of recombinant proteins for biotechnological and therapeutic applications at a large scale is an essential need of mankind. Huge application potential of therapeutic and industrial proteins has led to increasing demand for effective and efficient bioprocessing strategies. Recent progress around recombinant DNA technologies and bioprocessing strategies has paved the way for efficient production of recombinant proteins. Important factors such as insolubility and cost of production need to be considered for large scale production of these recombinant proteins. Fundamentals of Recombinant Protein Production, Purification and Characterization is organized into 9 chapters in a logical fashion beginning with an introduction to recombinant proteins and moving onto expression in different host expression systems followed by extraction, purification, and analysis of proteins. This important reference features protocols, along with the advantages and disadvantage of each expression hosts and characterization technique (presented in tabular format) and offers detailed coverage of all aspects of protein production and processing (upstream and downstream processing) in one place; and finally ends with different characterization techniques Includes step by step reproducible protocols while providing updated information on the rationale and latest developments in expression systems Can also be used as a handbook for protein expression and purification; as expression systems and chromatographic methods are explained in detail Consists of notes on troubleshooting from the eminent researchers in the field Provides comprehensive information on protein production, purification, and characterization in a single volume Describes different purification methods for comparatively difficult to obtain proteins Brings the topics of recombinant protein expression, purification and characterization together, thereby making it the first resource on how to solve problems with respect to upstream and downstream processing of heterologous proteins INDICE: 1. Introduction to recombinant protein expression and purification2. Recombinant protein expression in Escherichia coli3. Recombinant protein expression in Pichia pastoris4. Mammalian cells for recombinant protein production via transient gene transfer5. Expression of recombinant proteins in Chlamydomonas reinhardtii6. Extraction and concentration of proteins (Laboratory scale)7. Chromatographic and non-chromatographic techniques for purifying recombinant proteins8. Analysis for purity of recombinant proteins9. Characterization of recombinant proteins

• ISBN: 978-0-323-98388-4
• Editorial: Academic Press
• Encuadernacion: Rústica
• Páginas: 320
• Fecha Publicación: 01/07/2023
• Nº Volúmenes: 1
• Idioma: Inglés