Enzymes in RNA Science and Biotechnology, Part B, Volume 692 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on topics such as Quantitative base-resolution sequencing technology for mapping pseudouridines in mammalian mRNA, Quantitative base-resolution DAMM-seq for mapping RNA methylations in tRNA and mitochondrial dsRNA, Discovering RNA modification enzymes using a comparative genomics approach, Functional analysis of tRNA modification enzymes using mutational profiling, and Fluorescent labeling of tRNA for rapid kinetic interaction studies with tRNA-binding proteins.Other chapters cover Enzymatic Synthesis of RNA Standards for Mapping and Quantifying RNA Modifications in Sequencing Analysis, Characterizing RNase L-mediated mRNA decay in single cells, Characterization of RNase J, Pri-miRNA cleavage assays for the Microprocessor complex, The pre-miRNA cleavage assays for DICE, Methods for study of ribonuclease targeting chimeras, and Profiling the in vivo RNA interactome associated with the endoribonuclease RNase III in Staphylococcus aureus. Provides the authority and expertise of leading contributors from an international board of authorsPresents the latest release in Methods in Enzymology seriesUpdated release includes the latest information on Enzymes in RNA Science and Biotechnology INDICE: Post-transcriptional modification enzymes1. Quantitative base-resolution sequencing technology for mapping pseudouridines in mammalian mRNALi-Sheng Zhang2. Quantitative base-resolution DAMM-seq for mapping RNA methylations in tRNA and mitochondrial dsRNALi-Sheng Zhang3. Discovering RNA modification enzymes using a comparative genomics approachSatoshi Kimura4. Functional analysis of tRNA modification enzymes using mutational profilingRyota Yamagami and Hiroyuki Hori5. Fluorescent labeling of tRNA for rapid kinetic interaction studies with tRNA-binding proteinsUte Kothe and Sarah Schultz6. Enzymatic Synthesis of RNA Standards for Mapping and Quantifying RNA Modifications in Sequencing AnalysisHoward Gamper, Caroline McCormick, Amr Makhamreh, Meni Wanunu, Sara Rouhanifard and Ya-Ming HouNucleases7. Characterizing RNase L-mediated mRNA decay in single cellsJames Burke, Renee Cusic and Monty Watkins8. Characterization of RNase JBalasubramanian Gopal, Muralidharan Vandanashree and Ankur Singh9. Pri-miRNA cleavage assays for the Microprocessor complexTuan Anh Nguyen, Cong Truc Le and Thi Nhu Y. Le10. The pre-miRNA cleavage assays for DICERTuan Anh Nguyen, Cong Truc Le and Thi Nhu Y. Le11. Methods for study of ribonuclease targeting chimerasMatt Disney, Noah Springer, Samatha Meyer, Yuquan Tong, Jessica Childs-Disney and Raphael Isaac Benhamou12. Profiling the in vivo RNA interactome associated with the endoribonuclease RNase III in Staphylococcus aureusWinton Wu, Chi Nam Ignatius Pang, Jai Tree and Daniel Giuseppe Mediati
- ISBN: 978-0-443-23976-2
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 346
- Fecha Publicación: 06/11/2023
- Nº Volúmenes: 1
- Idioma: Inglés